The present study investigated the effect of zinc on the pigment content of Sorghum bicolor at different stages of plant development. The results revealed that increasing zinc treatment levels led to a rise in chlorophyll "a" content, with the highest increase observed at 7.5 mM (71.9%). Similarly, chlorophyll "b" content increased with higher zinc concentrations, reaching a maximum increase of 162% at 5.5 mM. Total chlorophyll content also showed an overall increase with increasing zinc levels, except at 1.5 mM where a slight drop of 0.52% was observed. Furthermore, the a/b ratio decreased as zinc concentration increased, indicating a potential sensitivity of chlorophyll "a" to elevated zinc levels. These findings are consistent with previous studies documenting the impact of zinc on pigment content in various plant species. The results suggest that zinc plays a crucial role in regulating pigment synthesis and photosynthetic processes in Sorghum bicolor. Further research is warranted to elucidate the underlying mechanisms and implications of zinc-induced changes in pigment content in plants