In the present study, pesticides are widely used in crop production and are known to induce major environmental problems in India. With an increased pesticide use, questions are rising on potential effects regarding public health, environment and soil health fertility. Pesticides pollute air, soil, water resources and contaminate the food chain. Which are essential catalyst rulings the quality of soil life. The role of pesticides viz; acephate and two pyridyl methyl amine class insecticide acetamiprid and imidacloprid on collected soil in winter season from different regions of Aligarh (Atrauli, Gabhana, Iglas, Khair and Koil). Results shown that the effects of pesticides on enzyme activity of dehydrogenase and urease enzymes slightly decrease while without pesticides enzyme activity of dehydrogenase and urease enzyme slightly increase