Simulation training has gained significant attention in the healthcare sector as an effective method for enhancing the skills and competencies of nurses and laboratory technicians in operation scenarios. This review article aims to critically analyze the effectiveness of simulation training programs for healthcare professionals, focusing specifically on nurses and laboratory technicians. The review synthesizes existing literature on simulation training in healthcare and its impact on improving clinical skills, decision-making abilities, teamwork, and patient outcomes. Various types of simulation modalities, such as high-fidelity simulators, virtual reality simulations, and standardized patients, are discussed in terms of their utility and effectiveness in training healthcare professionals. The review also examines the challenges and limitations associated with simulation training, including cost, time constraints, and fidelity issues. Strategies for optimizing simulation training programs to maximize learning outcomes and transferability of skills to real-world settings are explored. Additionally, the review highlights the importance of debriefing sessions following simulation exercises to enhance reflective learning and promote continuous improvement among healthcare professionals. Overall, the findings suggest that simulation training is a valuable tool for enhancing the competencies of nurses and laboratory technicians in operation scenarios. By providing a safe and controlled environment for practicing critical skills and decision-making, simulation training can help healthcare professionals improve their performance and confidence in high-pressure situations. Future research directions and recommendations for the implementation of simulation training programs in healthcare settings are discussed to guide policymakers, educators, and practitioners in leveraging this innovative educational approach.