Ulcerative colitis (UC) is one of the chronic inflammatory bowel diseases (IBD) that causes inflammation of colon mucosa and submucosa. This study aimed to investigate the effect of Pomegranate juice (PJ) and Apple juice (AJ) on body weight, blood chemistry and colon histology in rats with acetic acid-induced UC. Thirty-five mature rats were distributed into five groups (n=7 rats). Group 1 was kept negative control, group 2 was positive control with acetic acid-induced UC and groups 3, 4 and 5 with UC and orally given PJ, AJ and their combination for 6 weeks, respectively. Feed intake (FI), body weight gain (BWG %) and feed efficiency ratio (FER) were determined. Blood samples were collected for determination of serum aspartate aminotransferase (AST), alanine aminotransferase (ALT), uric acid (UA), creatinine (Cr.), total cholesterol (TC) and triglycerides (TG). Proinflammatory cytokines interleukin 1 beta (IL1β), IL6 and IL8 were measured. Glutathione peroxidase (GPx), superoxide dismutase (SOD) and catalase (CAT) were estimated. In colon homogenate, glutathione (GSH), malondialdehyde (MDA) and lactate dehydrogenase (LDH) were measured. Histopathological examination of colon was also performed. The results revealed that oral administration of combination of PJ and AJ to rats with UC significantly decreased body weight and serum levels of AST, ALT, UA, Cr., TC, TG and proinflammatory cytokines IL1β, IL6 and IL8. Combination of PJ and AJ increased serum GPx, SOD and CAT. It increased GSH, but decreased MDA and LDH in colon tissue homogenate. Histopathological examination of the colon showed that PJ and AJ together ameliorated inflammatory lesions of the colon in rats with UC. Therefore, drinking of PJ and AJ may be useful for patients who suffer from UC.