This study evaluated the effect of chia, sorghum, and their combination on diabetic rats. eight groups of six adult male albino rats, each weighing (150±10g), were formed from the 48 rats used in this investigation. for 28 days, 2.5 and 5%, respectively, of chia and sorghum seeds and their powdered mixes were given to the main diet. subcutaneous injections of alloxan (150 mg/kg body weight) were given to induce diabetic rats. samples were examined for biochemical markers 28 days after the experiment ended. diabetic groups resulted in a considerable drop in hdl whereas a large rise in tc, tg, vldl, ldl, u.a, creatinine, urea, got, gpt, alp, and glucose was observed. the results showed that diabetic rats fed with various experimental diets had improved in all previous parameters. a diet with 2.5% chia seeds outperformed one with sorghum seeds, while the best diet included a 5% mixture, indicating synergistic effect. this suggested that the first case's therapeutic outcomes were superior.