Nowadays, diseases associated with lipid accumulation in the human body such as obesity are becoming very important health issues. The aim of this study is to evaluate the impact of cafeteria diet feeding by Wistar rats, used as an experimental model of nutritional obesity, during 8 weeks, on lipid metabolism. Thus, we determined the levels of total cholesterol (TC) and triglycerides (TG) in plasma, lipoproteins and organs (liver, adipose tissue, muscle), and the activities of lipoprotein lipase (LPL) in organs, and hormone-sensitive lipase (HSL). The results show that cafeteria diet causes increased accumulation of lipids in adipose tissue leads to obesity with ectopic accumulation of lipids in other organs as liver, and induce lipoproteins metabolic disorders. Our results also show a disruption in the pathway of lipid storage enzyme (LPL) and lipid mobilization enzyme (HSL). Cafeteria diet is not only a primary risk for obesity, but also acts indirectly by adversely affecting other primary risk factors to serious chronic disease.