The current research looked to determine the impact of amiodarone, an antiarrhythmic medication, administered in multiple doses over the course of seven days on the hypoglycaemic action of rosiglitazone in normal and diabetic rats. Objective: The goal of the research was to identify the drug interaction between rosiglitazone and amiodarone in normal and diabetic rats. Method: These studies were conducted in six healthy and six diabetic rats of both sexes and the dose of the administered drugs was rosiglitazone 0.3 mg/kg and amiodarone 50 mg/kg body weight. The medication was ingested through the oral route. At predetermined intervals, blood samples were drawn from the tail vein, and a semi-autoanalyser was used to determine the amount of glucose by employing the GOD/POD method. Result: The findings showed that amiodarone, an antiarrhythmic medication, influenced the hypoglycaemic activity of rosiglitazone when given alone and in combination with rosiglitazone in normal and diabetic rats. Conclusion: According to this research, when amiodarone and rosiglitazone are used together, monitoring of the therapeutic drug is necessary for modifying the therapeutic dose