A rapid, accurate, selective, and sensitive RP-HPLC method has been developed and validated for estimation of moxifloxacin hydrochloride (MXF) and ketorolac tromethamine (KTA) in the bulk and ophthalmic formulation. Method: The chromatographic separation was achieved on ODS Phenomenex C18 column (250mm × 4.6mm, 5 μm). The compound was separated using mobile phase 0.025M Potassium dihydrogen phosphate solution: acetonitrile: methanol (30:35:35v/v/v). The pH of mobile phase was adjusted to 3.0 with ortho-phosphoric acid, flow rate was 1.0 ml/min and 25°C column temperature and detection wavelength was set at 300 nm for overall analysis. Results: The retention time of MXF and KTA was obtained at 2.67 and 5.39 min, respectively. The linearity study was performed in the concentration range of 2 to 20 μg/ml for MXF and KTA with correlation coefficient was found to be 0.999 for both drugs. The percentage purity of MXF and KTA was obtained in the range 98-102%. The limit of detection was 0.148 μg/ml and 0.272μg/ml and limit of quantification was 0.449 μg/ml and 0.824 μg/ml, respectively for MXF and KTA.