Mouth ulcer treatment is a challenging task for many civilizations of the world. There are different plants used for the effective treatment of mouth ulcers and aphthous. Drugs for mouth ulcers are obtained from different parts of the plants. Nobility of dosage forms with no side effects is a major development and thus extract of plant was used to prepare such types of dosage form. For the treatment of mouth ulcer and aphthous ulcer, extract of leaves of Solanum nigrum was taken for the study. Polymeric nanoparticles of the leaves extract of Solanum nigrum were prepared using ionic gellation method. Optimization of this formulation was done using 22 factoial designs. Characterization of nanoparticles containing Solanum nigrum leaves extract was done using some characterization evaluation parameters like zeta potential, FESEM studies. These prepared nanoparticles were then incorporated into chewing gum taken amount three factors and two levels. Response surface methodology (RSM) and central composite design (CCD) was used for the study. Medicated chewing gum were prepared and evaluated for the low and high levels of the ingredient variables.