Background: Dental age estimation may require its application on dead and the living. Forensic cases at medical examiner/coroner offices or clustering the victims in mass disasters are the most common scenarios, where dental age estimation is applied on the dead ; criminal and immigration cases are the ones where dental age estimation offers a scientific, inexpensive and reasonably fast means of age assessment. Objective: To determine the age estimation from second molar by modified gleiser and hunt method Materials and Method: The study sample consisted of 100 OPG’s (50 males and 50 females) of age ranging from 10 to 20 years with known date. The present study was conducted in the department of forensic odontology. The samples were collected from the department of oral medicine and radiology ,Saveetha dental college and hospital by using Planmeca software. The collected data was transferred to SPSS software for the result. Result: Using SPSS, The Standard deviation for Male 37 staging is ± 2.62 years. And, the Standard deviation for Female 37 staging is ± 2.58 years. The combination of Male and female tooth number 37 staging is revealing the good results with the standard deviation of ± 2.55 years Conclusion: Gleiser and Hunt developed a far more reliable technique of determining the dental and chronological age of a child and adolescent aged 10 to 20 years using a radiological method of permanent mandibular second molars.