The aim of this research is to study the effectiveness of Pseudomonas aeruginosa bacteria isolated from clinical samples to produce Protease, Lipase, Amylase, Urease, DNase and Hemolysine. One hundred samples were collected from patients hospitalized during July 2020 till the end of January 2021 to isolate and identify P. aeruginosa from clinical sources including infections of wounds and burns. Twenty three isolates were found to be belonging to P. aeruginosa, which mean an isolate rate of (23%) of the total samples. The diagnosis was carried out based on phenotypic characteristics and biochemical tests. P. aeruginosa was found in (10) out of (38) wound infections samples, (13) out of (62) burn infections samples. This means that isolates from the wound infections constituted the highest percentage of (26%) of the total samples, while the lowest percentage of (20.9%) was obtained from the burn infections samples. The results showed that (24.7%) of total isolates were Hemolysin producer on blood agar,It was also found that (60.8%) of total isolates were Amylase producer on Starch agar, (69.5%) of total isolates were Protease and Lipase producer on Skim milk agar and on Rhan agar, (21.7%) of total isolates were Urease producer on Urea agar and (30.4%) were DNase producer on a DNase agar medium.