Background: Researchers are employing plant extract in the nanoparticle synthesis process to meet the growing demand for environmentally friendly nanoparticles. As it is more affordable, environmentally benign, and readily scaled up for large-scale synthesis, green synthesis offers an improvement over chemical and physical methods. The necessity for metal nanoparticles produced biologically from plant extracts over other hazardous technologies is therefore a better alternative. The synthesized silver nanoparticles (AgNPs) from traditional medicinal plants such as Tridax procumbens possess superior biological properties yet the cytotoxicity has to be assessed. The present study aims to evaluate the cytotoxic activity of AgNPs synthesized using T. procumbens leaf extract. Materials and methods: In this study, silver nanoparticles from the leaf extract of T. procumbens were synthesized. The AgNPs were characterized using UV-visible spectrophotometer and then assessed its cytotoxic activity using the Brine Shrimp Lethality Assay (BSLA). Results: The biosynthesized AgNPs has shown decreased cytotoxic activity at all concentrations (5 μL, 10 μL, 20 μL, 40 μL, 80 μL) and may be used to create innovative nanoformulations for pathogenic oral diseases. Conclusion: Green synthesis of AgNPs utilizing T. procumbens possess lower cytotoxic activity and have more uses in treatment of oral diseases due to the growing demand for biosynthesized materials for various treatment modalities.