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ISSN 2063-5346
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CYTOTOXIC EFFECT OF TRIDAX PROCUMBENS STEM BASED CHITOSAN GEL AND COMMERCIAL WOUND HEALING GEL- AN IN VITRO STUDY

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Rieshy. V, M. Jeevitha, S. Rajeshkumar
» doi: 10.31838/ecb/2023.12.s2.055

Abstract

Introduction: Chitosan is a glucosamine (2-amino-2-deoxy—D-glucose) polymer with trace amounts of Nacetylglucosamine. Tridax procumbens L. is a perennial plant native to tropical America. A plant steroid called beta-sitosterol promotes epithelialization and wound healing. Cytotoxicity assays are critical because they aid in determining the intended biological usage. This current study is focused to evaluate and compare the cytotoxic effect of T. procumbens stem based chitosan gel and commercial wound healing gel. Materials and Method: The cytotoxic effect of T. procumbens stem based chitosan gel (test) and commercial wound healing gel (5 μL, 10 μL, 20 μL, 40 μL, 80 μL) at different concentrations was assessed by brine shrimp lethality assay. Result: The viability of the shrimp was analyzed for different concentrations for T. procumbens stem based chitosan gel and commercial wound healing gel. The results proved that T. procumbens stem based chitosan gel showed no cytoxicity after 24 h and reduced cytotoxicity after 48 h similar to commercial wound healing gel.. Conclusion: This study proves that the cytotoxic effect of T. procumbens stem based chitosan gel was comparable to commercial wound healing gel.. Therefore, T. procumbens stem based chitosan gel can be used as an alternative to commercial wound healing gel as it is more eco-friendly and a form of traditional medicine with no side-effects.

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