The Tris Thiourea cadmium chloride doped with L-Arginine (CTTCDA) was successfully grown into crystals using slow evaporation method. The grown crystals were the subject of numerous investigation, including UV-Visible spectra, FTIR, thermal analysis (TG-DTA), SEM-EDAX spectra, and powder X-ray diffraction. Spectral analyses in the UV-Visible region were used to determine the optical and electronic properties of (CTTCDA). The calculated band gap energy is 4.57 eV. By using FTIR analysis, the functional group of CTTCDA crystals doped with L-Tryptophan was observed. The CTTCDA is thermally stable up to 240 o C, according to TG-DTA studies. The powder X-ray diffraction pattern confirms the crystalline type and purity of the grown crystals. An analysis of grown crystals using a Scanning Electron Microscope reveals that they are of good quality and have a layer-like structure. The characterization studies have shown that synthesized crystals exhibit favorable properties for nonlinear optical applications.