Background: Aim: The aim of this study is to assess the knowledge and practices of critical care nurses regarding the prevention of ventilator associated pneumonia, in ICUs of Jinnah and Punjab Institute of Cardiology, and government hospitals of Lahore Pakistan. Materials and Methods: A descriptive cross-sectional study was conducted in different ICUs of Jinnah and Punjab Institute of Cardiology Hospital Lahore, Pakistan. Data was collected from 151 Nurses working in critical care units, through Purposive sampling technique. The Tool used for data collection was closed ended questionnaire distributed among participants. Study duration was four months. Results: The study was descriptive cross-sectional, so descriptive statistics was applied, frequency distribution was calculated, data normally was cheeked. The study shows that the participants with good knowledge was 62 (41.1%), fair knowledge were 54 (35.8%), and the participants with poor knowledge were 35 (23.2%). Similarly, Participants with good practices were 62 (41.1%), fair practices were 50 (33.1%), and the 39 (25.8%) were poor practices. Conclusion: The Study concluded that the majority of Nurses having good knowledge and practices regarding ventilator associated pneumonia. Background: Aim: The aim of this study is to assess the knowledge and practices of critical care nurses regarding the prevention of ventilator associated pneumonia, in ICUs of Jinnah and Punjab Institute of Cardiology, and government hospitals of Lahore Pakistan. Materials and Methods: A descriptive cross-sectional study was conducted in different ICUs of Jinnah and Punjab Institute of Cardiology Hospital Lahore, Pakistan. Data was collected from 151 Nurses working in critical care units, through Purposive sampling technique. The Tool used for data collection was closed ended questionnaire distributed among participants. Study duration was four months. Results: The study was descriptive cross-sectional, so descriptive statistics was applied, frequency distribution was calculated, data normally was cheeked. The study shows that the participants with good knowledge was 62 (41.1%), fair knowledge were 54 (35.8%), and the participants with poor knowledge were 35 (23.2%). Similarly, Participants with good practices were 62 (41.1%), fair practices were 50 (33.1%), and the 39 (25.8%) were poor practices. Conclusion: The Study concluded that the majority of Nurses having good knowledge and practices regarding ventilator associated pneumonia.