Background: A variety of significant macro- and micro- nutrient deficiencies may hinder the progression of periodontal diseases. This study aims to assess the correlation between salivary micronutrient levels in chronic periodontitis patients before and after non-surgical periodontal therapy. Materials and Methods: A total of 50 chronic periodontitis patients who reported to the Department of Periodontics, Saveetha Dental College and Hospitals, Chennai were enrolled. Levels of Zinc [Zn], Copper [Cu], Iron [Fe], and Selenium [Se] were measured from unstimulated saliva samples before and after scaling and root planing. Paired t-test was done and the results were considered to be statistically significant when the p-value was <0.05. Results: At baseline (before SRP), the levels of Zn, Cu, Fe and Se were 3.05± 0.53, 8.45± 0.73, 7.30± 2.21 and 2.2± 1.23 respectively. At 3 weeks follow-up (after SRP), the levels of Zn, Cu, Fe and Se were 4.95± 0.48, 5.95± 0.48, 5.74± 0.34 and 4.8± 1.01 respectively. The difference between levels of Zn, Cu, Fe and Se before and after nonsurgical periodontal therapy was statistically significant with p < 0.05. Conclusion: The salivary levels of Zn and Se were increased and levels of Cu and Fe were decreased in chronic periodontitis after non-surgical periodontal therapy. This indicates that the levels of micronutrients can be used as effective biomarkers for chronic periodontitis.