Emphasis on extensive study and research pertaining to neonatal pain management may emphatically reduce some of the adverse physiological and neurodevelopmental outcomes in neonates. Alleviation of pain is imperative for the overall wellbeing and improved physiological and neurological outcomes in neonates. Objective: To compare the analgesic effect of 24% Sucrose orally and breastfeeding in neonates during venipuncture. Material And Methods: 50 neonates in the Postnatal ward at KIMS Hospital, Bangalore, fulfilling the criteria were included in this study; These neonates were randomized into intervention groups (breast feeding, 24% sucrose). 2ml of sucrose solution was given to the baby by paladai 5 minutes before venipuncture and the other group of neonates were breastfed during venipuncture. The facial response, heart rate and oxygen saturation were monitored and recorded adhering to NIPS (Neonatal Infant Pain Scale). Result: Among 50 neonates, 25 of them were given 24% sucrose and 25 were breastfed. The study and analysis showed a significant lower score of Neonatal Infant Pain Scale (NIPS) in breastfed group (1.48) when compared to oral sucrose (2.08) at the end of 5 minutes. Conclusion: The findings of this study showed that breastfeeding caused reduction in pain compared to administering oral sucrose in neonates. Considering the fact that breastfeeding is a safe and free method accepted by mothers and health centre staff, it can be advised during painful procedures in order to reduce the infant’s pain. It also facilitates a mother to strengthen a renewed mother-child bond. Besides this, breastfeeding also provides psychological benefits for mothers as they partake in the infant’s care which does not invite any additional cost to the health care system.