Silk, a natural protein fiber, holds immense significance in society as one of the oldest and most versatile natural fibers worldwide. The fibroin protein present in silk can be dissolved in water, forming an aqueous solution that can be transformed into a desired form. This regenerated silk fibroin solution shows tremendous potential for various biomedical applications, including tissue engineering, regenerative medicine, and drug delivery. In this particular study, the objective was to solubilize fibroin extracted from three different sources, namely Daba(TV), Daba(BV), and Raily silk, using unconventional methods. The aim was to obtain silk fibroin powder that could be utilized as a valuable resource in the biomedical field, as well as in the cosmetics and food industries. To achieve this, pure silk fibroin protein was extracted from Daba(TV), Daba(BV), and Raily silk cocoons through a process called degumming. Aqueous Na2CO3 solution was used in this step. Subsequently, the silk fibroin was hydrolyzed in NaOH to obtain the desired silk powder.