Indoneesiella echioides (or) Andrographis echioides (L) Nees is an important herb widely distributed in south India. This is commonly known as False water willow. Indoneesiella echioides (L) Nees is used as in traditional Indian medicine. The leaf juice of this plant is used to cure fever. Different pharmacological properties of Indoneesiella echioides have already been reported. Thus, the present study was performed to investigate the preliminary phytochemical screening, separation, identification of compounds and compare the phytochemical composition of various fraction of Indoneesiella echioides using gas chromatography-mass spectrometry. The plant was extracted for various solvents in increasing order of polarity from using n-hexane, chloroform, ethyl acetate, acetone, ethanol, butanol and methanol. The result obtained after GC-MS studies were confirmed by spectral analysis.