There is a wide spectrum of severity in the mood disorder depression. Depression is caused by a disruption in the equilibrium of neurotransmitters. The present study was based in the comparative pharmacological screening of anti-depressant activity of different parts of Nyctanthes arbor-tristis plant in mice. Chemicals and drugs were obtained from the local market in Bareilly. The leaves and flower of the plant Nyctanthes arbor-tristis was collected in the month of April 2022 from Botanical Garden, MJPRU Bareilly and nearby area. Fresh plant materials were collected and authenticated by a Resident Botanist and Alok Srivastava, Professor (Department of Plant science), MJP Rohilkhand University, Bareilly. It was extracted using Soxhlet apparatus. Extract was characterized by phytochemical screening, TLC and UV analysis. From the IVRI in Bareilly, Uttar Pradesh, we choose Swiss albino mice weighing 30-40g. The protocol of the depressant activity investigation was approved by the Institutional Animal Ethical Committee.