The accuracy of the implant-supported prosthesis is related to the definitive cast (or “master cast”), who’s precision depends on the impression technique execution and dimensional stability of the splinting materials used. The purpose of this in vitro study was to evaluate the accuracy of the master cast using open tray impression technique and splinted with three splinting materials Bis-GMA temporizing material, light cure tray material, polyether bite registration material. A mandibular reference model with six implants was done. 33 custom trays were fabricated using the light curable resin sheets using medium body polyether impression material. These trays were randomly divided between the three groups, with eleven trays in each group. Impression techniques were divided into three groups namely: Group I: impression copings splinted with Bis-GMA temporizing material, Group II: impression copings splinted with light cure tray material, Group III: impression copings splinted with polyether bite registration material and then final impressions were made. Total of 33 master castswere fabricated. Optical microscope (OPUS 3020T MODEL) CMM, numerical difference in distance between the implants were evaluated. The difference among the three groups, one-way ANOVA, tukey HSD analysis and kruskal wallis test were performed. The results showed significant differences among the materials, as well as their interactions (P < 0.05). The master cast obtained by the splinting material Bis-GMA temporizing material, light cure tray material exhibits no difference from the reference model but deviation was found with cast obtained from polyether bite registration material. The Bis-GMA temporizing material and light cure tray material are easy to handle, less time consuming, less technique sensitive, rigid and has its routine utility in clinical practice.