The present study was conducted on the cow urine distillate (CUD) and ethanolic extracts of two plants of medicinal importance for exploring their therapeutic potential using the Ehrlich Ascites Carcinoma (EAC) mouse model. Ethanolic extracts of Catharanthus roseus (aerial parts) and Plumbago zeylanica (stem) were prepared and the presence of bioactive compounds was determined by spectrophotometric analysis and GCMS profiling. The antiangiogenic activity was determined using the chicken chorioallantoic membrane (CAM) model and EAC tumour mouse models were used to investigate the antitumour activity of the two extracts and CUD. Polyphenolic flavonoid content was found to be highest in P. zeylanica extract. GC MS profiling has also revealed the presence of esters and different sterols in the same. Antioxidant assays revealed maximum free radical scavenging potential in the C. roseus extract. The CAM model showed the CUD as a better suppressor of blood vessel growth than the extract of two plants as well as ethanol-treated control eggs. Similarly, CUD has demonstrated significant antitumour activity determined by the reduction of tumour size, volume and weight when compared with the highest doses of both the plant extracts. This study demonstrates that CUD has antiangiogenic and anticancer activities which can be utilized as a remedy against various tumourigenic cancerous diseases.