In this study, a brand-new, simple procedure for environmentally friendly silver nanoparticle and Clotrimazole production on wheat fibre was reported. The Polyethylene glycol-400 (PEG 400) was used in the liquid phase chemical technique to produce silver nanoparticles. PEG 400 was used in the manufacture of silver nanoparticles as a stabilizer and reducing agent. The typical silver nanoparticle is 150 nm in size. The produced silver nanoparticles may be distributed in water, ethanol, and other polar solvents, and they have promising uses in the electrical and biological sciences. Silver nanoparticle aggregation was reduced by using ethanol as a solvent. Clotrimazole was physically loaded onto cellulose fibre using a physical loading technique. The Wheat fibre received an effective antifungal property from the combination of Clotrimazole and silver nanoparticles. After washing, there was hardly any loss in the antifungal effectiveness of the cotton fabrics treated with nano silver and clotrimazole. As more silver nanoparticles were loaded onto the outer layers of the white Wheat fabrics, their colour altered to a yellowish brown. Additionally, the antifungal effectiveness of wheat fibre loaded with drugs and AgNP was assessed against the common fungus Candida albicans. The presence of Clotrimazole and silver nanoparticles on the wheat fibre was confirmed by the energy dispersive spectroscopy (EDS), scanning electron microscope (SEM), X-ray diffraction (XRD), and ultraviolet (UV) studies. The particle size analyzer determined the size of the silver nanoparticle