This study seeks to learn more about SnO2Pluronic F127 Chitosan Sodium. SnO2Pluronic F127 Chitosan NPs have been shown to have a tetragonal rutile structure by means of both X-ray diffraction and field-emission scanning electron microscopy. In the FTIR spectra of SnO2 NPs, the Chitosan functional group and the Pluronic F127 overtone bands are visible, while the characteristic peaks of SnO2 NPs are located at 619 cm -1 in the spectra of SnO. The morphologies of SnO2PluronicF127 Chitosan were analysed using scanning electron microscopy (SEM). SnO2Pluronic F127 Chitosan NPs have DLS spectra and hydrodynamic sizes of (169 nm), respectively. Due to oxygen vacancies and other defects, SnO2Pluronic F127 Chitosan NPs have blue emission peaks in their PL spectra, specifically at 467 nm. Antibacterial activity against Staphylococcus aureus, Klebsiellapneumoniae, Shigelladysenteriae, Pneumocystis jirovecii, Bacillus megaterium, and Proteus valgaris was reevaluated using the well-diffusion technique using SnO2Pluronic F127 Chitosan NPs and Amoxicillin.