Medicinal plants such as leaves of Neem have very unique therapeutics properties which made them used widely to treat a lot of human diseases. The present study were conducted characterize the crude extract of Neem and evaluate their biological activities. Neem leaves were extracted using hot-extraction method, and evaluated for their chemical group compounds using phytochemical screening for the chemical group compounds. Meanwhile, Gas chromatography (GC), Energy-dispersive X-ray spectroscopy (EDX) and Fourier-transform infrared spectroscopy(FTIR) were used to conform to the fictional groups in the leave of Neem. The antibacterial activities of the cured extract of Neemwere observed against two bacteria pathogens. Phytochemical screening analysis was done using common procedures and had shown the presence of alkaloids, carbohydrates, tannins, steroids, and flavonoids. Twenty-two compounds were identified in the GC-Mass spectrometry. The highest compounds were Pyridine,2,3,4,5-tetrahydro