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Chemical Composition and Scanning Electron Microscopic (SEM) Aspects of Uroliths in Geriatric Dogs

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Jupaka Shashank*,K. Satish Kumar,V.V.V. Amruth Kumar,B. Anil kumar,M. Lakshman,
» doi: 10.48047/ecb/2023.12.12.74


Out of the total 246 geriatric dogs with lower urinary tract disease (LUTD) in the present study, 71 dogs were diagnosed for the presence of uroliths of various size and shape and their location, using x-ray and ultrasonography. Out of these, the most prevalent anatomic locations of caliculi were in the urinary bladder, urethra in males, and majorly urinary bladder in females. The caliculi that were detected on x-ray, and ultrasonography were retrieved surgically and processed for chemical analysis, and subjected to scanning electron microscopy. Various caliculi that were investigated with scanning electron microscopy (SEM) revealed, perpendicularly cracked fragments showed concentric laminations composed of compact and loosely packed strata alternately as magnesium ammonium phosphate uroliths, surface of eggshell-like fragments exhibited the scattered hexa-hedral coffin lid-shaped crystals upon the numerous spherular crystals at center, towards periphery and periphery areas, irregularly arranged rock like structures as large and small sized, and large sized regular magnesium ammonium phosphate uroliths, surfaces of few calcium phosphates stones were cracked like egg shells, calcium oxalate monohydrate uroliths were noticed as ‘picket fence appearance’, and bipyramidal shape in calcium oxalate dihydrate crystals on scanning electron microscopy (SEM).

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