When compared to other traditional physical and chemical processes, the biosynthesis of metallic nanoparticles using plants, enzymes, and microorganisms has been recognised as an environmentally benign option. Scientists and researchers have recently shown a great deal of interest in the biological production of nanoparticles because of its straightforward method, low cost, non-toxicity, and environmental friendliness. Therefore, it was reported in this work that zinc oxide nanoparticles (ZnO-NPs) were synthesised utilising a reduction agent derived from Abelmoscus Manihot fruit pulp extract. Using an X-ray diffraction (XRD), UV-visabsorption spectroscopy, and a scanning electron microscope (SEM), the biosynthesized ZnO-NPs were characterised. It was discovered that the nanoparticles' average size fell between 30 and 57 nm. Using the agar diffusion method, ZnO-NPs were tested for their antibacterial effectiveness against harmful microbes. ZnO-NPs that were biosynthesized in this manner were found to have effective antibacterial activity against S. Typhi and E. Coli. In conclusion, the green ZnO-NPs were synthesised utilising Abelmoscus Manihot fruit extract, and remarkably, it was also demonstrated to have an antibacterial impact on strains of S. Typhi.