Bioinformatics which is a unique subject integration with biological and computational combined research to utilize the date of biology by computational tools to produce effective outcome in biomedical engineering. It is an interdisciplinary science that develops and improves upon methods storing, retrieving, organizing, and analyzing biological data. Research implication of computational tools, which bring utilization of different kinds of scientific data like medicine and health, life sciences, computer science, chemistry, statistics, mathematics, including those acquire, store, organize, archive, analyze. Multiple approach on bioinformatics brings a miracle change in research and development of any branch. It’s a big challenge for researchers to describe this infant field in a systematic scientific way and bring out the attention of its various applications and services. This article will present a broad-spectrum overview of bioinformatics, aims, applications, technologies, data produced in the biological world and how bioinformatics can organize, analyze, store, and discuss some of algorithms that can be implemented over bioinformatics data, and how to apply bioinformatics to discover, diagnosis of diseases and applications in biomedical by using computational tools.