This study underscores the development of Ag-PEG-Ch nanocomposite film using the aqueous leaves extract of Cannabis sativa under the influence of ultrasound, as smart substrates for antimicrobial uses, via innovative in situ reactive and reduction pathways. Ag nanoparticles that are synthesized by using leaves extract and ultrasound as an energy source have emerged as nontoxic and ecofriendly. In this study, silver nitrate was used as a silver precursor and PEG and chitosan were used as the polymeric stabilizer and solid support, while cannabis sativa leaves extract acts as a green reducing agent. This polymer metal film was characterized by FT-IR, SEM, TEM, TGA, DSC and antimicrobial activities. XRD peaks clearly reveals that FCC Ag nanoparticles embedded in polymer matrix, very fine Ag nanoparticles (4-6 nm) can be synthesized through ultrasound has been proven by TEM images and TGA-DSC results show that this nanocomposite film is thermally stable. This nanocomposite film possesses synergistic biocidal activities also.