The Rhizospheric soil bacteria are a group of bacteria which plays an important role in regulating organic matter, decomposition and nutrient cycle. They also play vital roles in plant nutrition, growth promotion and disease interaction. Hence these bacteria are very crucial for breeders and especially for leguminous crops. The present study was aimed to identify Rhizosphere soil bacteria using PCR based molecular markers like ISSR (Inter simple sequence repeats) markers and their biochemical characteristics. ISSR primer (UBC812) showed polymorphic amplification between the two samples thus proving that each sample contain different bacterial species. Further these samples were set for PCR reaction using 16s RNA primer which showed the conserved monomorphic amplification in all the samples. After sequencing and NCBI blast two bacteria were identified as (Acinetobacter calcoacetius, Bacillus arabhattai ). Identification of these bacteria will help breeders in future to create different types of biofertilizers which will increase the fertility of the soil, solubilization of minerals, fixation of nitrogen, production of growth promoting hormones and competitive suppression of pathogens in crops