The aim of the current study explore the properties of Azadirachta indica leaves (Family: Meliaceae) of Indian medicinal plant by phytochemical screening qualitatively, quantification their volatile compounds by gas chromatography and mass spectroscopy analysis (GCMS) and their antibacterial property against Vibrio cholera using MTCC strains V. cholera 3904 and 3906. In order to identify the potential biomolecules, methanolic (MeOH) extract was subjected to standard phytochemical methods. Qualitative analysis revealed the presence of alkaloids, total carbohydrates, cardiac glycosides, flavonoids, glycosides, phenols, saponins, and tannins.