Diabetes mellitus is one of the most challenging health problems of the 21st century and is a leading cause of death in developed countries. The treatment of urinary tract infection (UTI) in diabetics is defendant on the identification of the bacteria which is responsible for the cause and also the effective antibiotics against them. Treatment options for UTI in diabetes may be similar to as in non-diabetics but have to be initiated timely to prevent further complications and may take a longer time for cure. With increasing newer mechanism of resistance to various antimicrobials, prior knowledge of these causative agent in a particular hospital is very important. The study aimed to identify the bacteria responsible for the UTI in diabetic patients and also the antimicrobial susceptibility pattern. Objective: To find the prevalence of various Gram negative uropathogens in diabetic patients and antimicrobial resistance pattern among the isolated bacterial strains. Method: The urine sample were collected. Identification was done by Gram staining and various biochemical reactions after preparation, cultivation and isolation of urine sample. Antimicrobial susceptibility was done by Kirby Bauer disc diffusion method on Muller-Hinton agar plate. Result: The overall prevalence of UTI in diabetic mellitus patient was (54.44%) with the male predominance (62.24%) and in female with (37.75%). The UTI in diabetic patient was common in age 41-60 years (44.89%). E. coli (42.86%) was the most predominant bacterial isolate followed by Kl. pneumoniae (24.49%), Ps. aeruginosa (18.37%), Aci. baumannii (7.14%), Kl. oxytoca (5.10%) and Pro. mirabilis (2.04%). The antimicrobial susceptibility tests revealed that fosfomycin, tigecycline and nitrofurantoin showed maximum susceptibility for all urinary tract infection in diabetic patients. The overall prevalence of multidrug resistance (MDR) uropathogens was 88(88.77%). Conclusion: The prevalence of UTI and MDR to commonly used antibiotics among diabetic patients is alarming, and thus, isolation of uropathogenic bacteria and antimicrobial susceptibility testing is crucial for the treatment of UTI in persons with diabetes.