A potent bacterial strain (Bacillus spp.) was employed for the decolorization of effluent collected from textile industries. The present study includes biochemical characterization of the bacterium, physicochemical characterization of six distinct effluents (E1, E2, E3, E4, E5 and E6) and optimization of culture conditions for decolorization of effluent (E6) collected from the outlet of industrial area. The highest decolorization efficiency (74.8%) of bacterium was achieved within 90 hours of incubation. The effluent E6 collected from the main outlet of industrial area was highly polluted that have exhibited highest BOD, COD, DO, EC and TDS in comparison to other tested effluents. It was found that the pH 8, 35 °C temperature, 25% of bacterial inoculum and supplementation of glucose showed the optimal efficiency of effluent decolorization. Therefore, based on the result of the present study it can be explored that bacterial decolorization of effluent is very cost-effective and environment-friendly, which can be recommended for the decolorization of textile industry effluents containing carcinogenic dyes in large amounts, through industrial effluent treatment plant (ETP).