Objectives: The primary objective of this study is to assess the physicochemical and microbiological safety of the water supply in the main sources (water tank and tab water) of Sulaymaniyah city hospitals, as it directly impacts the health of patients. Methodology: This study involved collecting and analyzing water samples from twelve major hospitals in the city of Sulaymaniyah, Iraq. The samples were collected from the main sources of water and analyzed twice to ensure accuracy of data. The physical and chemical parameters assessed included pH, TDS, EC, hardness, as well as the concentrations of calcium, magnesium, chloride, sodium, nitrate, and sulfate. Microbiological analysis was also conducted using selective and differential chromogenic coliform agar (CCA) medium to count E. coli, total coliform, and heterotrophic bacteria in the water samples. Results: The physicochemical parameters of water samples in hospitals were found to be within international standards, indicating that the chemical and physical quality of the produced water did not pose any health risks. However, the microbiological results revealed that the levels of Coliform and E. coli contamination exceeded the permissible limit, which could potentially pose health concerns. Conclusion: Water samples in hospitals meet international standards for physicochemical parameters, but the presence of high levels of Coliform and E. coli contamination indicates potential health concerns related to the microbiological quality of the water produced. To address this issue, appropriate measures such as implementing proper water treatment protocols and regular monitoring are recommended to ensure safe consumption.