Dermatoglyphics has been found to be valuable in predicting anomalies related to the orofacial region. Aim: The current study aimed to evaluate the relation between fingerprints and spacing in primary dentition in children aged 3-6 years. Materials and Methods: A total of 320 children of age group 3-6 years were included in the study. Intraoral photographs were taken using a digital camera to assess spacing in the dentition. The ridge patterns of the left and right thumbs were recorded using a digital fingerprint scanner. Statistics: The correlation between dermatoglyphics and the incidence of spaced/ non-spaced dentition was assessed using the Shapiro-Wilk test. The Chi-square test was used to check the difference in proportions among several variables and to evaluate the data. Results: Subjects belonging to non-spaced and spaced dentition had more loops ridge patterns followed by whorls patterns in both right and left thumb fingerprints. All the variables had statistically significant values, with a degree of divergence of specific dermatoglyphic patterns among the left-hand participants. Conclusion: The study has shown that dermatoglyphic patterns could be used as a non-invasive anatomical tool for screening spacing in dentition and its correlation with malocclusion and guiding future research, concerning early diagnosis and instituting preventive and interceptive strategies