The present study was aimed to compare the role of oxidative stress markers in diabetic and senile cataract patients. A total of 135 patients were included in this study, of which, 50 patients had diabetic cataract while, remaining 85 were having senile cataract. Blood glucose concentration and serum oxidative stress markers like, xanthine oxidase (XOD), superoxide dismutase (SOD) and malondialdehyde (MDA) levels were estimated by using commercial available kits. HbA1c levels (%) estimated by using HPLC technique present in central diagnostic laboratory of SMI hospital. Fasting and post-prandial plasma glucose levels in diabetic cataract patients were significantly higher (p<0.001) as compared to the senile cataract patients. The activity of superoxide dismutase (SOD) was significantly decreased while Xanthine oxidase (XOD) activity and serum MDA levels were significantly increased in diabetic patients with cataracts in comparison to patients with senile cataracts (p<0.001). Serum XOD, SOD and MDA level was positively correlated with the HbA1c levels. We concluded that the hyperglycemic condition may associated with the increased production of reactive oxygen species (ROS) that trigger non-enzymatic glycation, increased production of lipid peroxidation products and advanced oxidative protein product which may contribute to the development of diabetic cataract. Therefore, the antioxidant therapy can delay the onset and progression of diabetic cataract.