The objective of the present study was to evaluate the protective effect of a polyherbal formulation developed against paracetamol and D‑galactosamine -induced hepatotoxicity in wistar rats. The research was conducted in two separate 10 and 14 day investigations against paracetamol and D-galactosamine, respectively. There were five distinct treatment groups for the animals. A toxicant group in two experiments received paracetamol 750 mg/kg orally every 72 hours for 10 days and D-galactosamine 400 mg/kg intravenously as a single dose. The test formulation was administered at doses of 100 mg/kg/day and 200 mg/kg/day. In addition to the D-galactosamine given to the toxicant group, test formulations were also administered to the treatment groups. The biochemical evaluation demonstrated that both paracetamol and galactosamine caused hepatotoxicity in the toxicant groups. Nevertheless, treatment with polyherbal formulation (PHF) significantly (P < 0.001, vs. toxicant) diminished the levels of SGOT, SGPT, serum bilirubin, and ALP, and decreased lipid peroxidation. In addition, treatment with the test formulation increased serum albumin and GSH levels significantly (P vs 0.001 vs. toxicant) compared to toxicant groups. On the premise of these studies and comparative evaluation, it can be concluded that PHF demonstrated hepatoprotective activity against paracetamol and D-galactosamine at 100 mg/kg and 200 mg/kg, respectively.