The diet pattern and level of hemoglobin are associated with the risk of the development of cardiovascular diseases. There are studies that explain the relationship between diet patterns and sleep. It is also said that there is an association between lack of sleep and obesity. Aim and objectives: The present study was undertaken to observe the hemoglobin content and sleep duration in vegetarians, and non-vegetarians. Materials and methods: All first-year Medical students were part of the study. Out of 150 students, Both males and females were part of the study. Unwilling students were excluded from the study. Hemoglobin in the blood samples was assessed using Sahli’s acid hemocytometry method which is a standard method in hematology. Diet pattern and sleep duration was assessed by personal interview method with the participants. Results: The mean weight of the participants is 60.24 kgs. The mean height of the participants is 164.3 cm. 44.9% of the participants were non-vegetarians. 50.6% of the participants sleep more than 6 hours. 90.8% of the participants do not use nutritional supplements. Both vegetarians and nonvegetarians have normal hemoglobin values. In comparison, non-vegetarians have higher levels of hemoglobin percentage. Conclusion: The study results are in accordance with earlier studies as we have observed higher hemoglobin in the non-vegetarians. The sleep duration was also high in the non-vegetarians. Further detailed studies are necessary for this area.