Type 2 diabetes's leading cause of mortality and morbidity mellitus (T2 DM) is the leading cause of cardiovascular disease and the expense associated with treating its effects. The present study was conducted to assess the association between time domain analysis of heart rate variability with glycated hemoglobin and duration of type II diabetes mellitus. Materials & Methods: 62 type II DM patients of both genders were put in group I and equal number of healthy subjects in group II. All subjects were subjected to measurement of 5 minutes ECG using Niviqure data acquisition system and time domain analysis of heart rate variability was done. HbA1c levels were estimated by high performance liquid chromatography. Results: The mean HbA1C (%) was 8.4 and 6.3, SBP (mm Hg) was 138.4 and 116.4, DBP (mm Hg) was 82.4 and 76.8, H.R. (beats/min) was 85.4 and 76.4, SDNN (ms) was 22.1 and 30.5, RMSSD (/min) was 19.4 and 22.3 and pNN50 (%) was 1.7 and 3.2 in group I and II respectively. We found that there was positive correlation in mean heart rate with both duration of diabetes and HbA1C. Conclusion: Heart rate variability can be used to screen for and detect cardiac autonomic neuropathy, which is caused by persistently increased blood sugar levels