In the current study, we investigated the efficacy of the active compounds present in Salvia hispanica and Avena sativa seeds in the treatment of osteoporosis on methylprednisolone in female rabbits. The study consists of two experiments; the first one is used to estimate the acute toxicity of chia( Salvia hispanica) and oat (Avena sativa) seeds ethanolic extracts. These results showed the seeds were safe up to 15000 mg/kg B.W dose. In accordance with this doses of 600 mg/kg B.W were selected for experimental study. The second experiment, 80 female rabbits were used, weighted 1500- 2000gm and aged 8 months, were divided into 8 equal groups. Group 1 served as control negative group(G-ve). Group2:Control positive group(G+ve) received S/C methylprednisolone(Meth-Pred.) of 0.2 mg/kg per for 1 month, then received orally 1ml/kg normal saline per day for 2 months. Group3(Meth-Pred+Sh): received S/C Meth-Pred. of 0.2 mg/kg for 1 month then received 600 mg/kg Salvia hispanica seed ethanolic extract orally per day for 2 months.Group4(Meth-Pred +As) : received Meth-Pred. for 1 month then received 600 mg/kg Avena sativa seed ethanolic extract orally for 2 months.Group5(MethPred+Alend): received Meth-Pred. for 1 month then treated by alendronate 3.6mg/kg.BW weekly orally for 2 months.Group6(Meth-Pred+ Alend + Sh): received Meth-Pred. of for 1 month then treated by alendronate 3.6mg/kg BW orally weekly and 600 mg/kg Salvia hispanica seed ethanolic extract orally per day for 2 months.Group7 (Meth-Pred+ Alend + As): received Meth-Pred for 1 month then treated by alendronate 3.6 mg/kg BW orally weekly and 600 mg/kg Avena sativa seed ethanolic extract orally per day for 2 months.Group8 (Meth-Pred+ Sh + As): received Meth-Pred. of for 1 month then treated by both Salvia hispanica seed ethanolic extract at 300 mg/kg and Avena sativa seed ethanolic extract at 300 mg/kg orally per day for 2 months. This study persisted till after 1 week of the medicine’s withdrawal, by studying the body weights, BMD, serum ALP, Calcitonin, Estradiol hormone, bone Ca, and phosphorus. The results showed that the body weights, BMD, Calcitonin, estradiol hormone, bone Ca, and phosphorus a significantly declined (p≤ 0.05), as well as a significant increase ( P≤0.05) in serum ALP of female rabbits after 2 months of treatment and after 1 week of withdrawal of treatment in control(+ve) and alendronate group compared with control(-ve), but when treated animals with Salvia hispanica and Avena sativa seeds ethanolic extracts in 3 and 4 group, demonstrated an improvement in the parameters above return to its normal values and produced good results. According to these results, Salvia hispanica and Avena sativa seeds are effective in preventing bone loss in female rabbits.