Present work is to investigate the possible application of produced biosurfactants for biodegradation of motor oil contaminated sand in laboratory. Pseudomonas aeruginosa was procured from Microbial Type Culture Collection Centre and Gene Bank for current investigation. The biosurfactant were produced using sludge as source of carbon, whichis low-cost substrates. Biosurfactant has the potential to treat and remediate petroleum sludge. Sludge is a solid or semisolid mass that settles down in the course of storage tanks. Oil field sludge consists of hydrocarbons,which includes mixtures along with asphaltenes and waxes. Moreover,sand, clays and water were also found in the sludge. Sludge generated by oil refining industry are hazardous waste and proper management and safe disposal is a major challenge for