Introduction: A wound is caused by skin injury from surgery, ulceration, cancer, wear and tear, and/or loss of the connective tissue beneath the skin. Green synthesis is a systematic method for producing a product without creating harmful byproducts. It is a long-term, effective, and replicable synthesis method. Tridax procumbens is a perennial weed taxonomically classified as a member of the Asteraceae family. Among other active components, T. procumbens's phytoconstituents contain sitosterol, carotenoids, luteolin, and linolenic acid. Beta-sitosterol, a plant steroid, aids in epithelialization and wound healing. The current study is focused on evaluating the antimicrobial effect of chitosan based T. procumbens stem gel. Materials and method: Chitosan based T. procumbens stem gel was prepared and assessed for antibacterial activity at various concentrations against pathogenic microorganisms, Staphylococcus aureus, Pseudomonas and Escherichia coli and compared with that of the commercial wound healing gel. The results were tabulated and graphically presented. Results: The Chitosan based T. procumbens stem gel exhibited higher antimicrobial activity than commercially available wound healing gel. The activity was dose dependent and was highest at 100 μL concentration. Conclusion: This study concluded that the antimicrobial effect of chitosan based T. procumbens stem gel is better than the commercially available wound healing gel and thus can be used as an alternative in pathogenic oral diseases.