This study examines the Lawsonia inermis (Henna) plant's ability to combat isolates amid wound swabs. Lawsonia inermis leaves were bought, spotted, and extracted by applying the cold maceration process with seventy percent (70%) ethanol. The plant excerpt was subjected to phytochemical screening applying a standard analytical technique. Patients, who gave their consent, had their wound surfaces containing purulent material, swabbed. Lawsonia inermis crude, chloroform, and aqueous extract were used on the isolates from the wound surfaces. Five plant metabolites, namely saponins, tannins, alkaloids, steroids, as well as terpenoids, were found using ethanol as extracting solvent. However, flavonoids and cardiac glycosides were not present. This excerpt had a smaller inhibitory zone compared to the positive control results with zones of 10.5±0.5mm and 10±0.00mm respectively, against M9N (S. aureus) and M9C (P. aeruginosa). The crude excerpt's greater efficacy was observed at 100 mg/ml. With zones of restraining of 10.5±0.5 and 9±1, respectively, M16N (S. aureus) and F3Y (S. aureus) revealed maximum action for the chloroform excerpt. With areas of inhibition of 8.5±0.5 and 11.0±0, respectively, M1C (P. aeruginosa) and F4Y (S. aureus) revealed the maximum action for the aqueous excerpt. The results of this study have provided support for the traditional medical uses of Lawsonia inermis in the development of brand-new antibacterial medications for the effective treatment of bacterial illnesses.