Reactive oxygen species (ROS) have been found to play an important role in the initiation and/or progression of various diseases, such as atherosclerosis, inflammatory injury, cancer, and cardiovascular disease. Antioxidants can protect the human body from damage caused by ROS and the concomitant lipid peroxidation, protein damage, and DNA strand breaking. Antioxidant and antihemolytic activities of various extract of BuchananialanzanSpreng leaf, and stem bark extract were investigated employing different in vitro assay systems. Ethanol extracts showed good antioxidant activity. Extracts exhibited good antioxidant activity in the haemoglobin-induced and also they were capable of scavenging hydrogen peroxide in a concentration dependent manner. Ethanol extracts showed good antihemolytic activity than other extract against hydrogen peroxide-induced hemolysis.