Introduction: Tridax procumbens, often known as coat buttons or tridax daisy, is a species of blooming plant that belongs to the daisy family. It is well known for being a widespread weed and nuisance plant. Although it is native to the tropical Americas, it has since expanded to regions with tropical, subtropical, and mild temperate climates. T. procumbens has long been used in India to treat wounds and as an insect repellent, antifungal, and anticoagulant. The main aim of this study is to evaluate anti-inflammatory activity of stem extract of T. procumbens based chitosan gel. Materials & methods: T. procumbens stem powder was prepared and the extract was collected. Medium molecular weight chitosan was added to the stem extract to prepare the wound healing gel. Anti- inflammatory activity of stem extract based chitosan gel at different concentrations (10 μL, 20 μL, 30 μL, 40 μL, 50 μL) was evaluated by Bovine serum albumin (BSA) & egg albumin assay (EA assay). Results: In BSA and EA assay, as the concentration increases from 10 μL to 50 μL the % inhibition also increases. The results showed that an increase in concentration increases anti-inflammatory activity. Conclusion: This study revealed that the stem extract of T. procumbens based chitosan gel had a potent antiinflammatory activity and can be used as a wound healing agent for oral applications.