To study the corneal epithelial thickness in dry eye disease (DED) eyes versus normal eyes using anterior segment optical coherence tomography along with its correlation with current dry eye diagnostics. Methodology- It was a cross-sectional study that took place from November 2021 to April 2022, enrolling 52 patients with symptomatic dry eye disease (DED) as cases and 52 healthy people as controls after thorough clinical examination, Schirmer’s test, Tear breakup time (TBUT) along with ocular surface disease index (OSDI) questionnaire. 208 eyes of 104 cases were subjected to Anterior segment Optical Coherence Tomography (AS-OCT) based Corneal epithelial thickness analysis. The corneal epithelial thickness in Central zone, Zone 1,2, &3 along with an average of 0 to 9 mm zone was calculated. Results- Corneal epithelial thickness in each zone was significantly higher compared to normal eyes. Schirmer’s test results and TBUT results have significant negative correlation to corneal epithelial thickness and OSDI score showed significant positive correlation to same. Conclusion- Increased epithelial thickness may be an objective clinical indicator of DED with potential to replace multiple invasive procedure like Schirmer and T-BUT for definitive DED diagnosis. AS-OCT is a simple non-contact approach offering fast, highly reproducible, quantifiable, precise corneal epithelial thickness. Corneal epithelial thickness can be incorporated in AI systems to screen for DED.