ISSN 2063-5346
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Neetu Prajapati , K. Nagarajan , Surbhi Kamboj , Parul Grover , Richa Goel , Sandeep Arora
» doi: 10.31838/ecb/2023.12.si6.091


In this study, a novel, rapid, simple, sensitive, and low-cost method was developed and validated by gas chromatography (GC) for the quantification of piperine. The GC analysis was performed on a capillary column [Column Rtx-5 (30 mm × 0.25 mm ID × 0.25 μm)], at a consistent flow rate of 1.3 ml/min and an injector temperature of 250 °C, nitrogen is utilized as the transporter gas with a split proportion of 50 with the flame ionization detection (FID). The injection volume was 10μl, and the run time was 20 minutes with a 16.7 retention time. All examinations were performed utilizing chemicals of analytical grade. The technique was validated according to the International Conference on Harmonization (ICH) guidelines for linearity, specificity, precision, the limit of detection (LOD), and the limit of quantification (LOQ). The standardization curve showed respectable linearity in the 50-150(ppm) series with a correlation coefficient of 0.999. The relative standard deviation (RSD) is not more than 2%. The method precision and intermediate precision were also within the acceptable range of RSD < 2%. The LOD and LOQ were 3.39% and, 9.99% respectively. The method was specific for piperine and did not show any interference from other components in the samples.

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