Gravure printing process is one of the dominant forms of printing processes being used for high quality flexible print products although, the printing process is capable of printing with 450 lpi, still a number of printing defects may happen during the print production. Objective of this paper is to through light on various printing defects happening in high-speed production-based Gravure printing presses. The data related to printing defect was collected on Rotomac printing press and printing defects were analysed on day-to-day basis for three months. The data was collected in observation basis the results indicated that mis-registration, doctor blade lines, shade variation (Each half tone and full tone) and grounding are the most frequently occurring defects and viscosity, continuity of track line, proper tension, impression roller pressure, temperature of heating zones, use of lay-on roller and use of inks are the least occurring printing defects. Over all print wastage found 6% because of various printing defects.