The Covid-19 pandemic has caused biochemistry practicum courses for biology education students to move online, leading to new difficulties. This descriptive qualitative study of 245 students from the 2018-2020 Batch in the biology education program at the University of Jambi found that students faced challenges with difficult biochemical materials, obtaining materials, and poor internet signals. Additionally, students struggled with comprehending the relationship between experimental processes and theory taught in class, and understanding practicum guidelines. The study underscores the need for solutions to improve biochemistry learning, particularly online, to enhance students' understanding of the subject matter