In the present study, we focused on one of the most expressive herbal plants, Aloe vera, which is unique for applications in general use and in treating various diseases. The evaluation of phytochemical components was based on molecular formula, molecular weight, retention time, and peak area. The obtained peaks were compared with the NIST library. The major phytochemical components revealed by GC-MS analysis of Aloe vera in the present study are 9-Octadecenoic acid, methyl ester (also known as Oleic Acid) (36.80%), Propanoic acid (8.16%), Ammonium acetate (7.20%), and 2, 3-Btanediol [S-(R*,R*)]- (4.19%); 6-Octadecenoic acid, methyl ester, (Z)- (3.39%); Phthalic acid, decyl isobutyl ester (3.30%); Methyl 9-cis,11-trans-octadecadienoate (2.89%); Heptadecanoic acid, 16-methyl-, methyl ester (2.63%); Hexadecanoic acid, methyl ester (2.21%); Ethanol,2,2’-oxybis- (1.97%); Acetic acid, methyl ester (1.86%); 2(5H)-Furanone, -methyl (1.62%); Glycerin (1.61%); Octanoic acid (n-Caprylic acid) (1.34%); Ethyl iso-allocholate (1.16%); 1,25-Dihydroxyvitamin D3, TMS derivative (0.67%); 9,12,15-Octadecatrienoic acid, 2,3-dihydroxy propyl ester, (Z, Z, Z)- (0.53%); Octaethylene glycol monododecyl ether (0.30%); Pterin-6-carboxylic acid (0.23%); Methyl 3-(acetyloxy)-20-hydroxyurs-12-en-28-oate (0.15%); Tetraacetyl-d-xylonic nitrile (0.05%) and Nitro-L-arginine (0.04%). The presence of the identified components suggests the anti-bacterial, anti-fungal, anti-microbial, anti-cancer, anti-tumor, anti-diabetic activities, anti-oxidant, analgesic, anti-inflammatory, wound healing potential, reproduction booster, and neuroprotective potential of the Aloe vera leaf gel.